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. 1982 Apr;150(1):395-7.
doi: 10.1128/jb.150.1.395-397.1982.

Phenotypic expression of mutations in a wide-host-range R plasmid in Escherichia coli and Rhizobium meliloti

P J Hooykaas, H den Dulk-Ras, R A Schilperoort

Phenotypic expression of mutations in a wide-host-range R plasmid in Escherichia coli and Rhizobium meliloti

P J Hooykaas et al. J Bacteriol. 1982 Apr.

Abstract

Eight different derivatives of R plasmid RP1 with thermosensitive mutations affecting maintenance in Escherichia coli and Pseudomonas aeruginosa were introduce into Rhizobium meliloti. None of the plasmids showed a thermosensitive character in R. meliloti. On the other hand, a certain deletion mutation in RP1 was found to cause plasmid instability in rhizobia and agrobacteria, but not in E. coli.

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