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. 1981 Oct 1;199(1):239-44.
doi: 10.1042/bj1990239.

The effect of limited proteolysis on rabbit muscle creatine kinase

N C Price, S Murray, E J Milner-White

The effect of limited proteolysis on rabbit muscle creatine kinase

N C Price et al. Biochem J. .

Abstract

Creatine kinase from rabbit muscle is inactivated by limited proteolysis with proteinase K from Tritirachium album. Gel-filtration and cross-linking studies showed that the limited proteolysis did not affect the molecular weight of the enzyme under non-denaturing conditions, but did cause changes in the reactivity of the reactive thiol group on each subunit and in the ability of the enzyme to form a 'transition-state analogue' complex in the presence of magnesium acetate plus ADP plus creatinine plus NaNO3.

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