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. 1982 Mar;79(5):1606-10.
doi: 10.1073/pnas.79.5.1606.

Hepatitis B virus core antigen: synthesis in Escherichia coli and application in diagnosis

S Stahl, P MacKay, M Magazin, S A Bruce, K Murray

Hepatitis B virus core antigen: synthesis in Escherichia coli and application in diagnosis

S Stahl et al. Proc Natl Acad Sci U S A. 1982 Mar.

Abstract

Fragments of hepatitis B virus DNA cloned in plasmid pBR322 carrying the gene for the viral core antigen have been placed under the control of the lac promoter of Escherichia coli. Several of the new recombinants direct higher levels of synthesis of the antigen, but the degree of enhancement varies with the different structures of the plasmids and hence the mRNAs produced. The antigen in crude bacterial lysates is a satisfactory diagnostic reagent for antibodies to the core antigen in serum samples.

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References

    1. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Bacteriol. 1972 May;110(2):667-76 - PubMed
    1. Nat New Biol. 1973 Jun 27;243(130):260-2 - PubMed
    1. Appl Microbiol. 1973 Oct;26(4):478-84 - PubMed

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