[Ultrastructural and cytochemical aspects of regenerating "Candida albicans" sphero-protoplasts (author's transl)]

Abstract

Subcellular modifications associated with sphero-protoplast regeneration of Candida albicans were followed with the electron microscope. After 30 min of regeneration the ultrastructural study revealed a considerable proliferation of plasmalemma invaginations and an abundant synthesis of glycogen in association with an extension of the Golgi apparatus/endoplasmic reticulum system. Later, the inflection of these invaginations and their fusion with the plasmalemma lead to a rejection of cytoplasmic areas in the cell wall. Glycogen-like particles so released in the wall were distributed in clearly delineated layers. The incorporation of cytoplasmic material through the intermediary of the plasmalemma constituted a mechanism believed to mediate the reconstitution and thus the integrity of the newly-forming cell wall.