Spread of R-plasmids among Escherichia coli causing urinary tract infections

Abstract

The incidence of multiple-antibiotic resistance among Escherichia coli isolated from urinary tract infections at Charing Cross Hospital, London, increased over the last 10 years, and its distribution was related to O-type. Among strains of the eight O-types most frequently causing such infections at this hospital, O4, O9, and O18 had a high incidence of multiple resistance (35, 22, and 19%, respectively); O2 and O6 had a intermediate incidence (14 and 11%, respectively); and O7, O1, and O75 had a low incidence (8, 6, and less than 3%, respectively). This nonrandom distribution appears to be a consequence of unequal plasmid recipient ability. After overnight mating with antibiotic-resistant donors, R-plasmid infection frequencies among antibiotic-sensitive urinary tract isolates differed by up to 10(5)-fold, and such differences were correlated with the variation in the incidence of antibiotic resistance among the O-types. The inherent differences in the ability to achieve significant R-plasmid spread, which appear to be determined by the host, not the plasmid, may be compounded in some cases by the inhibition of potential mating partners by colicin production.