Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982;27(2):138-41.
doi: 10.1007/BF02879773.

A rapid and economic method for estimation of the number of plasmid copies in Escherichia coli cells

J Hochmannová, J Nesvera

A rapid and economic method for estimation of the number of plasmid copies in Escherichia coli cells

J Hochmannová et al. Folia Microbiol (Praha). 1982.

Abstract

An economic method for a rapid estimation of the number of copies of plasmid R6K delta 1 in E. coli cells using lysis of the cells directly on the agarose gel and electrophoretic separation of radioactively labelled plasmid and chromosomal DNA's is described. The method, particularly useful for screening purposes, permits detection of both the CCC and OC forms of plasmids of molar mass 2-150 Mg/mol and it can be applied to other bacterial systems.

PubMed Disclaimer

References

    1. Mol Gen Genet. 1975 Sep 15;140(1):39-50 - PubMed
    1. Plasmid. 1978 Sep;1(4):584-8 - PubMed
    1. Nucleic Acids Res. 1977;4(5):1465-82 - PubMed
    1. J Bacteriol. 1976 Sep;127(3):1529-37 - PubMed
    1. J Bacteriol. 1977 Apr;130(1):148-53 - PubMed