Nitrocellulose immunofixation following agarose electrophoresis in the study of immunoglobulin G subgroups in unconcentrated cerebrospinal fluid

Abstract

A method is presented which enables the study of cerebrospinal fluid proteins in minimal volumes of unconcentrated fluid. The method involves agarose electrophoresis followed by transfer of the proteins to a nitrocellulose membrane and then demonstration of their distribution using a double-antibody immunoperoxidase technique. The method is used here for the study of immunoglobulin G and its subgroups, and clearly shows that the oligoclonal pattern observed in multiple sclerosis is not restricted to any one subgroup.