Analytical methods for thromboxane B2 measurement and validation of radioimmunoassay by gas liquid chromatography-mass spectrometry

Abstract

A sensitive and specific radioimmunoassay using a 125I tracer of high specific radioactivity was developed for thromboxane B2 and was applied to the determination of the in vitro biosynthesis of this compound in some systems (i.e. human washed platelets, human platelet rich plasma (PRP) and rat spleen homogenates). The assays were also evaluated by mass spectrometry; levels measured by these two analytical methods were very similar. The results obtained for washed human platelets with a thin layer radiochromatographic method were in good agreement with the two preceeding methods.