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. 1982 Apr;18(1):47-59.
doi: 10.1016/0378-1119(82)90055-5.

The nucleotide sequence of the HIS4 region of yeast

The nucleotide sequence of the HIS4 region of yeast

T F Donahue et al. Gene. 1982 Apr.

Abstract

We have determined the nucleotide sequence of the yeast HIS4 gene and its 5' and 3' flanking sequences. The protein chain has a calculated Mr-value of 87 935. Th 5' end of the HIS4 transcript maps at a position 63 bp upstream from the site of initiation of protein synthesis. The 3' end of the HIS4 transcript maps at a position approx. 118 bp after the UAG termination codon. There is no evidence for intervening sequences within the transcription unit. Functional sub-regions within the HIS4 coding frame have been identified by determining the sequence changes for various his4 mutations.

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