Effect of surface roughness on platelet adhesion under static and under flow conditions

Abstract

Normal blood clots when exposed to surfaces other than endothelial. Various unsuccessful attempts have been made to find a synthetic material that is compatible with blood. Both platelets and clotting factors are involved in thrombosis at foreign surfaces. The authors are concerned with platelet adhesion as a first step in thrombus formation. The determination of the number of platelets adhering per unit area, therefore, appears to be a useful criterion for the choice of appropriate biomaterials contacting blood. However, laboratory tests are often carried out with specially prepared, well-defined biomaterials with a smooth surface, whereas biomaterials in clinical use may have a variable degree of roughness. In this paper the authors present data on platelet adherence to a hydrophilic (glass) and to a hydrophobic (silane) material, with smooth and rough surfaces. Additional data are presented that document the extent of platelet adhesion to a wide range of smooth polymer materials having a large variation in surface hydrophobic quality. There was no difference in platelet adherence between the smooth and rough surfaces when tested under static conditions. When the surfaces were tested in a laminar flow cell, the addition of roughness caused a decrease in platelet adhesion on the hydrophilic surface and an increase in platelet adhesion on the hydrophobic surface.