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. 1982 Jan 8;227(1):25-31.
doi: 10.1016/s0378-4347(00)80352-7.

Improved procedure for the separation of phospholipids by high-performance liquid chromatography

S S Chen, A Y Kou

Improved procedure for the separation of phospholipids by high-performance liquid chromatography

S S Chen et al. J Chromatogr. .

Abstract

We describe a rapid and efficient high-performance liquid chromatography procedure for the separation of phospholipids. The separation is accomplished on a microparticulate silica gel column using isocratic elution and UV detection at 203 nm. The solvent mixture is acetonitrile--methanol--85% phosphoric acid(130:5:1.5, v/v). Complete separation is achieved within 30 min of phosphatidylinositol, phosphatidylserine, phosphatidylethanolamine, phosphatidyldimethylethanolamine, lysophosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine and sphingomyelin. The method is suitable for the analysis of phospholipids in tissue extracts.

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