Rapid determination of ammonia in whole blood and plasma using flow injection analysis
- PMID: 7060277
- DOI: 10.1016/0009-8981(82)90399-0
Rapid determination of ammonia in whole blood and plasma using flow injection analysis
Abstract
A flow injection method for the determination of ammonia in whole blood and plasma is described. The method utilizes diffusion of ammonia into a stream of a pH-sensitive indicator, which is monitored by a photometer. A volume of 90 microliter is needed and the result is obtained within 1 min. Ammonia concentrations increase during storage due to deamination. It is proposed to use plasma samples, which could be stored frozen at - 20 degrees C without significant change.
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