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. 1982 Feb 12;227(2):331-9.
doi: 10.1016/s0378-4347(00)80387-4.

Determination of gamma-aminobutyric acid by liquid chromatography with electrochemical detection

W L Caudill, G P Houck, R M Wightman

Determination of gamma-aminobutyric acid by liquid chromatography with electrochemical detection

W L Caudill et al. J Chromatogr. .

Abstract

gamma-Aminobutyric acid (GABA) has been determined in rat brain by derivatization with 2,4,6-trinitrobenzenesulfonic acid. The derivative and an internal standard, 2,4,6-trinitrophenyl-delta-aminovaleric acid, are extracted into toluene and separated by reversed-phase chromatography. Electrochemical reduction of these derivatives permits picomole measurements of GABA in microgram amounts of brain tissue.

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