The localization of vasotocin and neurophysin neurons in the diencephalon of the pigeon, Columba livia

Abstract

Vasotocin (VT)- and neurophysin (NP)-synthesizing neurons were demonstrated by immunocytochemistry in the diencephalon of the pigeon, Columba livia. Three diencephalic regions contain VT-NP cells: (1) periventricular preoptic area and hypothalamus, including nucleus periventricularis magnocellularis (PVM); (2) lateral preoptic area and hypothalamus; and (3) dorsal diencephalon. The immunoreactive cells in each of these three regions were divided into groups based on cytology and topography. No differences were found in the location of VT and NP cell groups. The periventricular region contains three continuous cell groups (P1-P3) extending from the posteroventral preoptic area to the anterodorsal hypothalamus and PVM. The lateral region has two cell groups composed of medium- to large-sized cells associated with the quintofrontal tract (L1) or with the optic tract (L2), while a third group (L3) lies between these two cell groups. Two accessory cell groups reside in the dorsolateral hypothalamus; L4 contains scattered cells of varied size, whereas L5 has small- to medium-sized cells clumped together. The dorsal diencephalic cell groups are found in the following locations: (1) lateral and dorsal to the lateral forebrain bundle (DD1); (2) in the area ventral to the dorsomedial anterior thalamic nucleus and dorsolateral to PVM (DD2); and (3) at the dorsolateral border of nucleus rotundus (DD3). To avoid potentially inaccurate mammalian homologies, the cell group nomenclature denotes topographic position. Nevertheless, the presence of VT-NP cells in PVM and projections to the brainstem and spinal cord suggest a homology between PVM and some of the parvocellular subnuclei of the mammalian paraventricular nucleus.