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. 1982 Feb;30(2):183-4.
doi: 10.1177/30.2.7061820.

High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry

High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry

F Gallyas et al. J Histochem Cytochem. 1982 Feb.

Abstract

A simple and reliable method is described for the intensification of the end-product of the diaminobenzidine reaction demonstrating peroxidase activity. After completing the diaminobenzidine reaction, the preparations to be intensified are immersed first in thioglycolic acid solution, then in distilled water, and finally in a special physical developer containing silver nitrate.

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