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. 1982 Apr;30(4):401-2.
doi: 10.1177/30.4.7061832.

A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range

D Pette, H Reichmann

A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range

D Pette et al. J Histochem Cytochem. 1982 Apr.

Abstract

A method is described for quantitative extraction of soluble enzyme activities from tissue samples in the milligram range. Mechanical disintegration of the frozen tissue is performed in a micromortar from which the powdered tissue can be quantitatively transferred for extraction in small volumes within a microcentrifuge tube.

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