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Comparative Study
. 1982;71(3):403-7.
doi: 10.1016/0305-0491(82)90401-1.

Interactions of different albumins and animal sera with insolubilized Cibacron Blue, Evaluation of apparent affinity constants

J Naval, M Calvo, F Lampreave, A Piñeiro
Comparative Study

Interactions of different albumins and animal sera with insolubilized Cibacron Blue, Evaluation of apparent affinity constants

J Naval et al. Comp Biochem Physiol B. 1982.

Abstract

1. A high concentration Cibacron Blue-Sepharose derivative has been used to study the affinity chromatography of albumin from eight animal species. 2. The apparent affinity constants for albumin varies between 3.9 x 10(4) M-1 and 0.9 x 10(4) M-1, in the order: Human greater than rabbit greater than horse greater than pig = dog greater than bovine greater than rat greater than chicken. 3. Other serum proteins were also bound to the gel, particularly lipoproteins and alpha 2-macroglobulin.

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