Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 May;150(2):973-6.
doi: 10.1128/jb.150.2.973-976.1982.

Cadmium and manganese transport in Staphylococcus aureus membrane vesicles

R D Perry, S Silver

Cadmium and manganese transport in Staphylococcus aureus membrane vesicles

R D Perry et al. J Bacteriol. 1982 May.

Abstract

The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ and Cd2+ were competitive inhibitors of each other's transport, with Km's of 0.95 microM Mn2+ and 0.2 microM Cd2+. The kinetic parameters were nearly identical with vesicles prepared from sensitive and resistant cells, indicating that the cadA-encoded Cd2+ efflux system was inoperative in membrane vesicle preparations. Experiments with energy-inhibited cells indicated that the cadB gene product may bind Cd2+.

PubMed Disclaimer

References

    1. J Bacteriol. 1968 Apr;95(4):1335-42 - PubMed
    1. J Biol Chem. 1971 Oct 10;246(19):5857-61 - PubMed
    1. J Bacteriol. 1972 Nov;112(2):761-72 - PubMed
    1. J Biol Chem. 1972 Dec 10;247(23):7452-8 - PubMed
    1. Antimicrob Agents Chemother. 1975 Jan;7(1):8-14 - PubMed

Publication types

LinkOut - more resources