Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Apr;93(1):230-6.
doi: 10.1083/jcb.93.1.230.

Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction

E J Patzer, D M Schlossman, J E Rothman

Release of clathrin from coated vesicles dependent upon a nucleoside triphosphate and a cytosol fraction

E J Patzer et al. J Cell Biol. 1982 Apr.

Abstract

Calf-brain coated vesicles were incubated with ATP and a cytosol fraction. As much as 90% of the clathrin was selectively released within 10 min at 37 degrees C without detectable proteolysis. This uncoating process required the presence of both ATP and cytosol. Empty cages of clathrin could also be dissociated in a similar manner. A nonhydrolyzable analogue, 5'-adenylylimidodiphosphate (AMP-PNP), would not substitute for ATP. Clathrin was dissociated from coats in a form unable to reassemble into cages under standard conditions. These reactions may reflect a segment of a clathrin-coated vesicle cycle in which coats are removed from vesicles after budding.

PubMed Disclaimer

References

    1. J Cell Biol. 1973 May;57(2):315-44 - PubMed
    1. J Mol Biol. 1975 Sep 5;97(1):93-8 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Cell. 1977 Mar;10(3):351-64 - PubMed
    1. J Cell Biol. 1977 Oct;75(1):135-47 - PubMed

Publication types