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. 1977 Jul;74(7):2840-3.
doi: 10.1073/pnas.74.7.2840.

Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields

J C Myers, S Spiegelman, D L Kacian

Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields

J C Myers et al. Proc Natl Acad Sci U S A. 1977 Jul.

Abstract

The synthesis of full-length complementary DNA copies of avian myeloblastosis virus RNA is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a molecular weight of 2.6 X 10(6), equivalent in size to that of the RNA template. Most (60-70%) of the reaction product is the full-length material and yields correspond to 60-70% of the input RNA.

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References

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    1. Biochemistry. 1975 Aug 26;14(17):3787-94 - PubMed
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