doi: 10.1073/pnas.74.7.2840.
Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields
- PMID: 70785
- PMCID: PMC431313
- DOI: 10.1073/pnas.74.7.2840
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Synthesis of full-length DNA copies of avian myeloblastosis virus RNA in high yields
Proc Natl Acad Sci U S A.
1977 Jul.
Abstract
The synthesis of full-length complementary DNA copies of avian myeloblastosis virus RNA is described. The cDNA is estimated by electrophoresis in agarose-methylmercury gels to have a molecular weight of 2.6 X 10(6), equivalent in size to that of the RNA template. Most (60-70%) of the reaction product is the full-length material and yields correspond to 60-70% of the input RNA.
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