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Comparative Study
. 1982 Mar 25;10(6):2121-34.
doi: 10.1093/nar/10.6.2121.

Analysis of difference spectra of protonated DNA: determination of degree of protonation of nitrogen bases and the fractions of disordered nucleotide pairs

T I Smol'janinova, V A Zhidkov, G V Sokolov
Free PMC article
Comparative Study

Analysis of difference spectra of protonated DNA: determination of degree of protonation of nitrogen bases and the fractions of disordered nucleotide pairs

T I Smol'janinova et al. Nucleic Acids Res. .
Free PMC article

Abstract

The titration curves of nitrogen bases and fractions of disordered nucleotide pairs are obtained during DNA protonation. It is shown that purine bases are the first sites of the DNA double helix protonation. The cytosine protonation is due to proton-induced conformational transition within GC pairs with the sequence proton transfer from (N-7) of guanine to (N-3) of cytosine. Within DNA with unwound regions the bases are protonated in the following order: cytosine, adenine, guanine. It is shown that GC pairs are the primary centres in which the unwinding of protonated DNAs occurs.

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