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. 1982;74(1):57-61.
doi: 10.1007/BF00495052.

A reliable preparation of mon-dispersed chromosome suspensions for flow cytometry

M Stoehr, K J Hutter, M Frank, K Goerttler

A reliable preparation of mon-dispersed chromosome suspensions for flow cytometry

M Stoehr et al. Histochemistry. 1982.

Abstract

A reproducible, simple and rapid protocol was developed to prepare mono-dispersed chromosome suspensions for flow cytometric analysis. The procedure basically employes: 1. twice rinsing of monolayer cultures to eliminate dead cells and cellular debris, 2. mild fixation of mitotic cells with 1% acetic acid, and 3. ultra sonic treatment to release single metaphase chromosomes. The procedure takes less than 30 min. Isolated chromosomes are storable over months at 4 degrees C. Despite mild fixation many biochemical studies and experiments applied on such fixed chromosomes purified and enriched using electronic sorting are feasible: 1. gene and restriction mapping, 2. cloning of specific gene sequences, and 3. gene frequency analysis.

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References

    1. J Histochem Cytochem. 1981 Jan;29(1):74-8 - PubMed
    1. J Histochem Cytochem. 1979 Jan;27(1):445-53 - PubMed
    1. J Histochem Cytochem. 1979 Jan;27(1):564-6 - PubMed
    1. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1231-4 - PubMed
    1. J Histochem Cytochem. 1979 Jan;27(1):441-4 - PubMed

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