How to analyze binding, enzyme and uptake data: the simplest case, a single phase

Abstract

The results of many biochemical, pharmacological and physiological experiments fit a rectangular hyperbola. Several methods for analysis of this curve have been developed but these techniques have not been readily available to researchers who are not interested in becoming mathematical statisticians. We have reviewed these analytical methods and concluded that, the Eadie-Hofstee technique is the best choice. We explain how to use this method and include sample calculations and calculator programs. We are especially interested in showing scientists who are not particularly mathematically inclined how to use these methods effectively and thus save a great deal of time and expense.