doi: 10.1016/0005-2795(78)90053-3.
Studies on cephalopod rhodopsin. Fatty acid esters of sucrose as effective detergents
- PMID: 708776
- DOI: 10.1016/0005-2795(78)90053-3
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Studies on cephalopod rhodopsin. Fatty acid esters of sucrose as effective detergents
Biochim Biophys Acta.
.
Abstract
Squid rhodopsin was extracted with solutions of fatty acid esters of sucrose (monolaurate and monostearate) and purified by DEAE-cellulose and concanavalin A-Sepharose affinity chromatography. The purified rhodopsin (A280/A480 = 2.5) contained 2.3 mol of glucosamine and 1.2 mol of phospholipid per mol of rhodopsin. The photoproduct metarhodopsin was also stable in these detergent solutions as in digitonin solution. Concanavalin A had no affinity for retinochrome.
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