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. 1978 Sep 26;536(1):78-87.
doi: 10.1016/0005-2795(78)90053-3.

Studies on cephalopod rhodopsin. Fatty acid esters of sucrose as effective detergents

K Nashima, M Mitsudo, Y Kito

Studies on cephalopod rhodopsin. Fatty acid esters of sucrose as effective detergents

K Nashima et al. Biochim Biophys Acta. .

Abstract

Squid rhodopsin was extracted with solutions of fatty acid esters of sucrose (monolaurate and monostearate) and purified by DEAE-cellulose and concanavalin A-Sepharose affinity chromatography. The purified rhodopsin (A280/A480 = 2.5) contained 2.3 mol of glucosamine and 1.2 mol of phospholipid per mol of rhodopsin. The photoproduct metarhodopsin was also stable in these detergent solutions as in digitonin solution. Concanavalin A had no affinity for retinochrome.

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