Complete amino acid sequence of human brain calmodulin

Abstract

The complete amino acid sequence of calmodulin from human brain has been determined by using peptides derived from digests with trypsin. Staphylococcus aureus V8 protease, and cyanogen bromide. The peptides were purified by means of reversed-phase high-performance liquid chromatography and analyzed with a sequenator. The protein contains 148 amino acid residues and has a molecular weight of 16792. As in other calmodulins, the amino-terminal residue of the protein is blocked by an acetyl group, and a trimethyllysine residue is located at position 115. The only difference between this sequence and those fully determined in other species is the assignment of amide groups.