Synthesis and composition of glycosaminoglycans by cultured human trabecular meshwork cells

Abstract

A human trabecular meshwork cell line with a limited number of population doublings was established in monolayer culture. All cultures produced hyaluronic acid, heparan sulfate, chondroitin sulfate, and dermatan sulfate. Following [14C]-glucosamine incorporation into proliferating (phase II) cultures, 70%--80% of the medium glycosaminoglycan label was found in hyaluronic acid and 8%--14% in heparin sulfate. About 60% of the cell-bound activity was associated with hyaluronic acid and 30% with heparan sulfate. Long-term cultivation under nondividing ("senescent") conditions resulted in a change of the pattern of synthesized and excreted (medium) [14C]-glucosamine-labeled glycosaminoglycans with a relative decrease of hyaluronic acid and a relative increase of heparan sulfate.