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. 1982 May 25;10(10):3249-60.
doi: 10.1093/nar/10.10.3249.

Syringe method for stepwise chemical synthesis of oligonucleotides

T Tanaka, R L Letsinger
Free PMC article

Syringe method for stepwise chemical synthesis of oligonucleotides

T Tanaka et al. Nucleic Acids Res. .
Free PMC article

Abstract

A simple procedure is described for synthesis of oligonucleotides by phosphate chemistry. Chains can be constructed rapidly with minimal equipment (a syringe and reagent bottles). The method is illustrated by synthesis of d-TGCAGGTT. Pertinent supporting data on the effect of variations in the detritylation, condensation, oxidation, capping and cleavage steps in the synthetic approach and in isolation procedures are also presented.

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