Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1982 May;131(3):179-83.
doi: 10.1007/BF00405875.

Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase

Comparative Study

Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase

K Motosugi et al. Arch Microbiol. 1982 May.

Abstract

The occurrence of a new bacterial dehalogenase acting on both the optical isomers of 2-halogenated alkanoic acids was demonstrated. When the haloalkanoic acid-utilizing bacteria were screened in a medium containing DL-2-chloropropionate as a sole carbon source, two types of bacteria were isolated: (1) a few strains utilizing both D- and L-isomers of 2-chloropropionate and (2) strains utilizing only the L-isomer. A dehalogenating enzyme was obtained from the cells of Pseudomonas sp. which is able to utilize both isomers. The crude enzyme catalyzed the dehalogenation of D- and L-2-chloropropionates to yield L- and D-isomers of lactate, respectively. The enzyme showed the same pH optimum and heat inactivation rate for the D- and L-isomers. Apparent Km values for D- and L-2-chloropropionates were 4.5 and 1.0mM, respectively. The enzyme acted specifically on 2-haloalkanoic acids. Activity staining of disc-gels electrophoresed with the crude enzyme preparation showed that the dehalogenation of D- and L-2-chloropropionates, monochloroacetate, dichloroacetate, 2,2-dichloropropionate, and DL-2-chlorobutyrate is due to a single protein.

PubMed Disclaimer

References

    1. Eur J Biochem. 1971 Jul 15;21(1):99-109 - PubMed
    1. J Biol Chem. 1968 Jan 25;243(2):428-34 - PubMed
    1. Ann N Y Acad Sci. 1964 Dec 28;121:404-27 - PubMed
    1. Can J Microbiol. 1959 Jun;5(3):255-60 - PubMed
    1. Nature. 1976 Oct 7;263(5577):476-9 - PubMed

Publication types

LinkOut - more resources