Activity and modulation of ornithine decarboxylase and concentrations of polyamines in various tissues of rats as a function of age

Abstract

The activities of ornithine decarboxylase (ODC) on the soluble and nuclear fractions of the cerebral cortex, heart and lungs of 4- (young), 38- (adult) and 85-week (old) male rats were studied. Also, the effects of aminophylline, histamine and estradiol on the activity of soluble ODC have been determined in vitro using slices of these tissues. The activity of ODC is significantly higher in the soluble fraction of all the tissues in comparison to that of nuclear fraction. Its activity in both the fractions is highest in the immature and decreases with increasing age in all the tissues except in the nuclear fraction of the lungs in which it increases with age. The ODC of the heart, lungs and cerebral cortex appear to be different as seen from the differences in their sensitivities to aminophylline, histamine and estradiol. In general, there is a decrease in its sensitivity to the three effectors with increasing age. This may be due to a decrease in the receptors and a concomitant decrease in ODC activity. A direct relationship between ODC activity and polyamine levels of the brain exists at various ages of the rat.