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. 1982 Apr;18(1):29-37.
doi: 10.1016/0378-1119(82)90053-1.

Ribosomal protein genes of yeast contain intervening sequences

G H Bollen, C M Molenaar, L H Cohen, M M van Raamsdonk-Duin, W H Mager, R J Planta

Ribosomal protein genes of yeast contain intervening sequences

G H Bollen et al. Gene. 1982 Apr.

Abstract

From a colony bank of HindIII-generated yeast DNA fragments we have isolated a number of recombinant DNAs carrying genes for ribosomal proteins (e.g., S10, S16A, S20, S24, S31, S33, L16, L25 and L34) of the yeast Saccharomyces carlsbergensis. By electron microscopic analysis of the R-loops formed between various DNA fragments and yeast mRNA, we could locate the ribosomal protein genes on the physical maps of the cloned DNA fragments. The R-loop structures observed indicate that a number of the ribosomal protein genes contain an intervening sequence.

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