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. 1982 Jul;16(1):209-11.
doi: 10.1128/jcm.16.1.209-211.1982.

Comparison of various techniques for determining viability of Paracoccidioides brasiliensis yeast-form cells

A Restrepo, L E Cano, C de Bedout, E Brummer, D A Stevens

Comparison of various techniques for determining viability of Paracoccidioides brasiliensis yeast-form cells

A Restrepo et al. J Clin Microbiol. 1982 Jul.

Abstract

The viability of Paracoccidioides brasiliensis yeast-form cells was determined by colony-forming units, direct fluorescent staining, and production of germ tubes in slide culture. The first procedure was unreliable and time consuming; the latter two showed better correlation with hemacytometer total cell counts and required significantly less time.

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