Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Jun;3(2):145-60.
doi: 10.1007/BF00711939.

A radioimmunoassay method for quantification of alpha-tropomyosin in heart homogenates

P B Moore, L F Lemanski

A radioimmunoassay method for quantification of alpha-tropomyosin in heart homogenates

P B Moore et al. J Muscle Res Cell Motil. 1982 Jun.

Abstract

A new, extremely sensitive, solid phase radioimmunoassay has been developed to quantify tropomyosin levels in heart tissue homogenates. Specific antibody was coupled to Sepharose 4B and saturation levels of [125I]tropomyosin bound. Release of radiolabel into the supernatant portion occurred when heart homogenates, authentic tropomyosin or tropomyosin in the presence of homogenate were added to these immunobeads. Quantification of the amount of tropomyosin was based on the level of release effected by standard tropomyosin with and without homogenate. The assay was determined to be highly specific and sensitive for tropomyosin. Picomolar quantities of the protein were readily detectable. Linearity extended well over the range of 5-200 ng tropomyosin in the homogenate. The method can be applied to other proteins for quantification during embryonic development.

PubMed Disclaimer

References

    1. J Biol Chem. 1978 Feb 25;253(4):1129-36 - PubMed
    1. Biochem Biophys Res Commun. 1979 Jul 12;89(1):181-7 - PubMed
    1. J Biol Chem. 1978 Feb 25;253(4):1137-48 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Biochemistry. 1971 Jun 22;10(13):2606-17 - PubMed