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Comparative Study
. 1982 Jun 25;10(12):3781-9.
doi: 10.1093/nar/10.12.3781.

5,6-Saturated thymine lesions in DNA: production by ultraviolet light or hydrogen peroxide

B Demple, S Linn
Free PMC article
Comparative Study

5,6-Saturated thymine lesions in DNA: production by ultraviolet light or hydrogen peroxide

B Demple et al. Nucleic Acids Res. .
Free PMC article

Abstract

Thymine analogs with saturated 5-6 bonds are important types of DNA damage that are recognized by the DNA N-glycosylase activity of E. coli endonuclease III. Seeking agents which could preferentially form 5,6-hydrated thymine residues in duplex DNA both in vivo and in vitro, we exposed purified duplex DNA to 325- or 313-nm light; however, after such exposure pyrimidine dimers greatly predominated over 5,6-hydrated thymine. Hydrogen peroxide, on the other hand, formed significant numbers of endonuclease III-sensitive sites in vitro which were not apurinic/apyrimidinic lesions and thus were likely to be 5,6-hydrated thymines.

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