[Staining with hematoxylin--basic fuchsin-picric acid (HBFP) for detection of myocardial lesions of different etiology]

Abstract

The article discusses advantages of the so-called fuchsinorrhagic method offered by Lie et al. in 1971. Areas of the necrotized muscle obtained at autopsy of 11 individuals who had died of myocardial infarction of various periods of duration, 12 biopsy specimens of the myocardium obtained during the operation for correction of congenital heart defects of the type of Fallot's tetrad and isolated defects of the septum with various periods of extracorporeal circulation and hearts of 35 male Wistar rats with a damaged myocardium (lesions being induced by intro-abdominal injection of noradrenalin in a dose of 2.5 mg/kg), which were sacrified 6 hours following the exposure to noradrenalin, were studied. The method of staining with HBFP makes it possible to detect both ischemic and noncoronarogenic lesions of the myocardium; moreover, in this method erythrocytes, fibrin, and connective structures get also stained, which makes a complex study of morphology of the myocardium feasible.