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. 1978 Oct;136(1):441-3.
doi: 10.1128/jb.136.1.441-443.1978.

Lack of lysogenic induction in "diaminopimelic acid spheroplasts"

A Bailone, A Levine

Lack of lysogenic induction in "diaminopimelic acid spheroplasts"

A Bailone et al. J Bacteriol. 1978 Oct.

Abstract

As part of an attempt to develop a semi-in vitro system of lysogenic induction, using spheroplasts of Escherichia coli K-12 lysogenic for prophage lambda, we prepared spheroplasts by depriving E. coli dap of diaminopimelic acid (DAP-spheroplasts). DAP-spheroplasts made from E. coli (lambda cI857) were thermally inducible. However, DAP-spheroplasts of E. coli (lambda) were not inducible by UV light. Thus, it appears that a functional cell wall is required for UV induction of prophage lambda.

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References

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