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. 1982 Feb;73(1):124-8.

An improved short-term culture method for human mammary epithelial cells

M Hiratsuka, T Senoo, T Kimoto, M Namba
  • PMID: 7117742

An improved short-term culture method for human mammary epithelial cells

M Hiratsuka et al. Gan. 1982 Feb.

Abstract

An improved short-term culture method for human mammary epithelial cells was developed and the details are presented. Briefly, after the digestion of biopsy tissues with 0.2% collagenase solution for 16 to 18 hr, 4 consecutive fractions were obtained by differential sedimentation. The last fraction, S-4, contained organoids having alveolar- or duct-like structures and gave rise to mammary epithelial cell growth with minimal contamination of fibroblasts.

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