doi: 10.1515/bchm2.1982.363.2.771.
Improved purification and N-terminal amino acid sequence determination of the contact site A glycoprotein of Dictyostelium discoideum
- PMID: 7118072
- DOI: 10.1515/bchm2.1982.363.2.771
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Improved purification and N-terminal amino acid sequence determination of the contact site A glycoprotein of Dictyostelium discoideum
Hoppe Seylers Z Physiol Chem.
1982 Aug.
Abstract
On aggregating cells of Dictyostelium discoideum a specific glycoprotein is expressed which is absent from growth phase cells of this organism. The glycoprotein has been related to cell surface antigens, called contact sites A, that are defined by their reaction with aggregation blocking antibody fragments. In the present paper an improved purification scheme for the glycoprotein is described and its N-terminal sequence is given. The purification has been monitored by use of a monoclonal antibody. The contact site A glycoprotein has the properties of an integral membrane protein.
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