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. 1982 Aug;16(2):355-60.
doi: 10.1128/jcm.16.2.355-360.1982.

Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography

G O Guerrant, M A Lambert, C W Moss

Analysis of short-chain acids from anaerobic bacteria by high-performance liquid chromatography

G O Guerrant et al. J Clin Microbiol. 1982 Aug.

Abstract

A standard mixture of 25 short-chain fatty acids was resolved by high-performance liquid chromatography, using an Aminex HPX-87 column. The acids produced in culture media by anaerobic bacteria were analyzed by high-performance liquid chromatography after extraction with ether and reextraction into a small volume of 0.1 N NaOH. The presence of fumaric acid in culture extracts of Peptostreptococcus anaerobius was confirmed by gas chromatography-mass spectrometry analysis of the trapped eluent fractions from the high-performance liquid chromatography column.

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References

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