Variations in the time course of the synchronization of intercostal motoneurones in the cat

Abstract

1. Synchronization of intercostal motoneurones was studied by the construction of cross-correlation histograms which related the firing times of paired groups of efferent inspiratory or expiratory discharges recorded from filaments of the external or internal nerves of anaesthetized or decerebrate cats.2. The principal feature of the histograms was always a central peak but the time course of the central peak showed considerable variation. Three forms of synchronization were defined on the basis of the time course of the central peak: (i) short-term synchronization (Sears & Stagg, 1976), where the peak was narrow, extending over about +/-3 ms but sometimes with weak shoulders to about +/-5 ms; (ii) broad-peak synchronization where the peak was wider than this (often +/-20 ms or more) but where there were no strong periodicities; (iii) high-frequency oscillation (h.f.o) synchronization, which was named from the related phenomena in medullary and phrenic recordings (Cohen, 1979), where there were periodic peaks on either side of the central peak with a frequency in the range 60-120 Hz. Combinations of these forms of synchronization were seen in some histograms.3. When different animals were compared, broad peak synchronization was seen in association with light anaesthesia and with polysynaptic excitation of the motoneurones from muscle spindle afferents.4. In individual animals, additional anaesthesia depressed both broad peak and h.f.o. synchronization.5. Raising P(A, CO2), which increased the respiratory drive to the motoneurones, favoured short-term or h.f.o. synchronization at the expense of broad-peak synchronization.6. In three decerebrate animals only short-term or h.f.o. synchronization was seen.7. Spinal cord lesions above or below the segments of interest promoted broad-peak synchronization, even with high P(A, CO2) or deep anaesthesia.8. We conclude: (i) that short-term synchronization, due mainly to the branching of presynaptic axons, is generated mainly by those axons which transmit the respiratory drive, that drive providing most of the excitation of the motoneurones in moderately deep anaesthesia; (ii) that h.f.o. synchronization arises from the periodic synchronization of the discharges in these same presynaptic axons; (iii) that broad-peak synchronization is generated by the activity of other presynaptic neurones whose discharges are also synchronized, but aperiodically, these neurones most likely including spinal cord interneurones which are active in light anaesthesia or when released by spinal cord lesions.9. These conclusions are supported by comparisons between intracellular recordings from inspiratory motoneurones in animals showing different forms of motoneurone synchronization, the comparison including the measurements of ;average common excitation' (a.c.e.) potentials (Kirkwood & Sears, 1978).