Increased proliferation of oligodendrocytes in the hypomyelinated mouse mutant-jimpy

Abstract

Previous studies of the hypomyelinated mouse mutant jimpy have shown that the number of oligodendrocytes are reduced about 50%. To determine the cause of the cellular reduction, light and electron microscopy were combined with thymidine autoradiographic techniques. The number of neuroglial cells which incorporate radioactive thymidine in the mutants is increased severalfold over control values. Electron microscopic autoradiograms indicate the majority of the labeled cells are oligodendroblasts. However, the total number of glia in the white matter of jimpy and control animals is the same during development and even up to the time of the animal's death. The presence of mitotic cells suggest that the oligodendrocytes undergo division but the abundance of dying cells suggests that they die sometime afterwards. The results of the quantitative autoradiographic studies in combination with our other data strongly suggest that the immediate failure of these cells to form myelin sheaths is due to a shortened life span and/or continued cell proliferation.