A colcemid-sensitive mechanism involved in regulation of chromosome movements during meiotic pairing

Abstract

Active movements of the chromosomes may be needed in the process, where homologous chromosomes find each other during the meiotic pairing. Because the components of the cytoskeleton are generally believed to be responsible for all movements in living nonmuscle cells, we have analyzed the regulation of the movements of zygotene chromosomes in the male rat by using specific inhibitors of the assembly of the various components of the cytoskeleton. --Colcemid, an inhibitor of microtubule formation, completely inhibited the chromosome movements in vitro at a concentration of 1 microgram/ml. This was associated with a damage of the nuclear envelope revealed by the electron microscopic analysis. Another inhibitor of microtubule formation, vinblastine, was ineffective below the level of general toxicity (100 microgram/ml). A specific microfilament inhibitor, cytochalasin B was similarly ineffective. --The findings suggest the presence of a specific colcemid-sensitive mechanism in the nuclear envelope of the zygotene spermatocytes, which regulates the movements of the chromosomes during meiotic pairing.