Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982;104(1-2):53-61.
doi: 10.1007/BF00402053.

Effect of diazepam on microsomal diethylnitrosamine metabolism in gerbils

P Conradt, U Green

Effect of diazepam on microsomal diethylnitrosamine metabolism in gerbils

P Conradt et al. J Cancer Res Clin Oncol. 1982.

Abstract

Dealkylation of diethylnitrosamine (DEN) by a liver microsomal fraction was measured after treatment of the Mongolian gerbil with DEN, Valium, or DEN plus Valium. Valium-dosed male and female animals showed strongly stimulated deethylase levels compared with the controls. This was also true of the DEN-treated females. Combined treatment (DEN + Valium) resulted in a significant loss of activity compared with treatment with DEN only, but was not significantly lower than that in the controls. Significant differences were also found between the experimental groups in microsomal enzymes. Metabolism of 14C-DEN to 14CO2 by gerbil liver slices in vitro showed dose-dependent inhibition by Valium. Kinetic analysis of DEN-dealkylation by purified gerbil microsomes revealed at least two Km values. In the microsomal system, diazepam inhibited DEN-dealkylation significantly at low substrate levels.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Ackermann R, Richter K (1977) Diazepam metabolism in human foetal and adult liver. Eur J Clin Pharmacol 11:43–49 - PubMed
    1. Albro PW (1975) Determination of protein in preparations of microsomes. Anal Biochem64:485–493 - PubMed
    1. Arcos JC, Bryant GM, Pastor KM, Argus MF (1976) Structural limits of specificity of methylcholanthrene-repressible nitrosamine N-dealkylases. Inhibition by analog substrates. Z Krebsforsch 86:171–183 - PubMed
    1. Autrup H, Harris CC, Trump BF (1978) Metabolism of acyclic and cyclic N-nitrosamines by cultured human colon. Proc Soc Exp Biol Med 159:111–115 - PubMed
    1. Baginsky ES, Foa PP, Zak B (1970) Glucose-6-phosphatase. In: Bergmeyer U (ed) Methoden der enzymatischen Analyse I. Verlag Chemie, Weinheim/Bergstrasse (pp 468–73)

LinkOut - more resources