Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Sep;94(3):662-6.
doi: 10.1083/jcb.94.3.662.

Ultrastructure of free ribonucleoprotein complexes in spread mammalian nuclei

R G Tsanev, L P Djondjurov

Ultrastructure of free ribonucleoprotein complexes in spread mammalian nuclei

R G Tsanev et al. J Cell Biol. 1982 Sep.

Abstract

Mouse erythroleukemia cell nuclei obtained by three different methods were spread for electron microscopy under low ionic conditions. It was found that this procedure allows the observation of free large ribonucleoprotein (RNP) complexes released from the nuclei during the centrifugation. The morphology of these complexes was readily affected by the conditions of cell treatment and spreading. Two extreme forms of free nuclear RNP structures were obtained, both consisting of spherical particles with diameters of approximately 17-20 nm. The first type was of loosened complexes of irregularly assembled particles interconnected with RNA fibrils. The second represented tightly packed particles forming mostly branched structures. The latter structures appeared to be closer to the native form of the nuclear RNP particles, differing from polyribosomes by their characteristic branching and stability in EDTA solutions.

PubMed Disclaimer

References

    1. Exp Cell Res. 1967 Apr;46(1):65-77 - PubMed
    1. J Mol Biol. 1968 Apr 14;33(1):251-63 - PubMed
    1. J Ultrastruct Res. 1969 May;27(3):266-88 - PubMed
    1. Biochemistry. 1969 Jul;8(7):3000-5 - PubMed
    1. Acta Endocrinol Suppl (Copenh). 1972;168:155-77 - PubMed