Abnormal distribution of proteins in the soleus and extensor digitorum longus of dystrophic mice

Abstract

Proteins of the whole muscle homogenates of the slow-twitch soleus (SOL) and fast-twitch extensor digitorum longus (EDL) of normal and dystrophic C57BL/6J mice at 4, 8, 12, and 32 weeks of age were resolved on polyacrylamide isoelectric focusing gels. Gels of the normal SOL proteins at all ages contained two bands specific to SOL and not represented in EDL. Gels of normal EDL contained three bands highly amplified in EDL but barely detectable in SOL. The distribution of proteins in dystrophic SOL was abnormal at all age groups studied due, in part, to a decrease in the proportion of SOL-specific proteins relative to other proteins in the muscle. The distribution of proteins in dystrophic EDL appeared abnormal first at 12 weeks due to a decrease in the relative proportion of EDL-amplified proteins. Due to these and other changes, at 32 weeks the dystrophic SOL and EDL were almost indistinguishable on the basis of their proteins' distributions.