Morphology and enzymatic capacity in arm and leg muscles in 78-81 year old men and women

Abstract

Twelve men and twelve women 78-81 years of age were studied with muscle biopsies from the right vastus lateralis and biceps brachii and with measurements of isometric and isokinetic strength for knee-extention and isometric strength for elbow-flexion. Bicycle ergometry with determination of heart rate and oxygen uptake at submaximal (50 W) and "maximal" work loads was also performed. Body cell mass was estimated from measurements of total body potassium. Muscle fibre composition with respect to slow twitch (ST = type I) and fast twitch (FT = type II) fibres did not differ between the sexes and the younger subjects drawn from population studies. The mean fibre areas averaged in vastus lateralis 4.7 and 3.3 micrometers 2 X 10(3) in men and women, respectively. This is less than 10 up to 30% of values found in sedentary younger subjects. The decline dominated in FT fibres, especially FTb fibres. In contrast biceps brachii did not show any matched fibre size reduction. The number of atrophic fibres was high and so was the frequency of "enclosed" fibres and areas with type grouping (ST fibres), indicating denervation--reinnervation. Such abnormalities are rarely seen in younger ages. Correlation analysis showed that only a minor part of the reduction in body cell mass with age could be explained by a reduction in fibre areas and that a reduction with age of the number of muscle fibres of both fibre types must be assumed. Positive correlations were observed between muscle strength and mean fibre and FT fibre areas. Comparing the present findings of skeletal muscle morphology to those in younger age groups, it is apparent that during the 8th decade of life major changes do occur. The measurements of aerobic and anaerobic enzymatic activities and of muscle capillary supply showed that levels comparable to those of younger age groups are maintained. Thus, quantitative rather than qualitative changes may explain the reduction in work performance with age.