doi: 10.1159/000459095.
Radiochemical assay for gamma-aminolevulinate synthase
- PMID: 7140714
- DOI: 10.1159/000459095
Item in Clipboard
Radiochemical assay for gamma-aminolevulinate synthase
Enzyme.
1982.
Abstract
We describe here a standard protocol for the radiochemical assay of gamma-aminolevulinate synthase from 17-day chick embryo liver mitochondria and starved male rat liver mitochondria. The assay is sensitive to at least 15 pmol of the gamma-aminolevulinate produced and can be adapted to suit most tissue types. A number of possible modifications to the procedure are discussed.
Similar articles
-
An improved method of radiochemical assay of delta-aminolevulinate synthetase and its application to the study of the level of hepatic delta-aminolevulinate synthetase in normal rat.Tohoku J Exp Med. 1973 Nov;111(3):297-304. doi: 10.1620/tjem.111.297. Tohoku J Exp Med. 1973. PMID: 4791097 No abstract available.
-
Purification of hepatic mitochondrial 5-aminolevulinate synthase.Methods Enzymol. 1986;123:395-401. doi: 10.1016/s0076-6879(86)23048-7. Methods Enzymol. 1986. PMID: 3702735 No abstract available.
-
Radiochemical microassay of delta-aminolevulinic acid synthetase in hepatic and erythroid tissues.Anal Biochem. 1972 Jun;47(2):457-70. doi: 10.1016/0003-2697(72)90139-x. Anal Biochem. 1972. PMID: 5036456 No abstract available.
-
[Control of the amount of delta-aminolevulinate synthase in liver mitochondria (author's transl)].Seikagaku. 1979 Jun;51(6):420-36. Seikagaku. 1979. PMID: 226633 Review. Japanese. No abstract available.
-
Regulation by heme of synthesis and intracellular translocation of delta-aminolevulinate synthase in the liver.Mol Cell Biochem. 1981 Jun 9;37(1):27-41. doi: 10.1007/BF02355885. Mol Cell Biochem. 1981. PMID: 6789140 Review. No abstract available.
Cited by
-
Measurement of 5-aminolaevulinate synthase activity by gas-liquid chromatography with electron-capture detection.Biochem J. 1990 Aug 15;270(1):103-7. doi: 10.1042/bj2700103. Biochem J. 1990. PMID: 2396971 Free PMC article.
-
delta-Aminolaevulinate synthase in human HepG2 hepatoma cells. Repression by haemin and induction by chemicals.Biochem J. 1989 Sep 15;262(3):807-13. doi: 10.1042/bj2620807. Biochem J. 1989. PMID: 2556111 Free PMC article.
-
A method for determining δ-aminolevulinic acid synthase activity in homogenized cells and tissues.Clin Biochem. 2015 Aug;48(12):788-95. doi: 10.1016/j.clinbiochem.2015.04.023. Epub 2015 May 8. Clin Biochem. 2015. PMID: 25959086 Free PMC article.
-
The effects of acute-phase inducers and dimethyl sulphoxide on delta-aminolaevulinate synthase activity in human HepG2 hepatoma cells.Biochem J. 1989 Apr 15;259(2):605-7. doi: 10.1042/bj2590605. Biochem J. 1989. PMID: 2541694 Free PMC article.
-
Biphasic ordered induction of heme synthesis in differentiating murine erythroleukemia cells: role of erythroid 5-aminolevulinate synthase.Mol Cell Biol. 1993 Nov;13(11):7122-32. doi: 10.1128/mcb.13.11.7122-7132.1993. Mol Cell Biol. 1993. PMID: 8413301 Free PMC article.