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. 1982;28(2-3):109-19.
doi: 10.1159/000459095.

Radiochemical assay for gamma-aminolevulinate synthase

J D Brooker, G Srivastava, B K May, W H Elliott

Radiochemical assay for gamma-aminolevulinate synthase

J D Brooker et al. Enzyme. 1982.

Abstract

We describe here a standard protocol for the radiochemical assay of gamma-aminolevulinate synthase from 17-day chick embryo liver mitochondria and starved male rat liver mitochondria. The assay is sensitive to at least 15 pmol of the gamma-aminolevulinate produced and can be adapted to suit most tissue types. A number of possible modifications to the procedure are discussed.

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