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. 1982 Sep 30;53(3):313-9.
doi: 10.1016/0022-1759(82)90178-8.

One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography

C Bruck, D Portetelle, C Glineur, A Bollen

One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography

C Bruck et al. J Immunol Methods. .

Abstract

Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.

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