doi: 10.1016/0022-1759(82)90178-8.
One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography
- PMID: 7142712
- DOI: 10.1016/0022-1759(82)90178-8
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One-step purification of mouse monoclonal antibodies from ascitic fluid by DEAE Affi-Gel blue chromatography
J Immunol Methods.
.
Abstract
Monoclonal antibodies can be purified directly from ascitic fluids by chromatography on a DEAE Affi-gel blue column. Optimal conditions were determined for the recovery of immunoglobulins free of contaminating protease and nuclease activities.
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