doi: 10.1038/300598a0.
Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein
- PMID: 7144911
- DOI: 10.1038/300598a0
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Construction of influenza haemagglutinin genes that code for intracellular and secreted forms of the protein
Nature.
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Abstract
The DNA sequences encoding the amino-terminal signal peptide or the carboxy-terminal hydrophobic anchor have been deleted from a cloned gene coding for the haemagglutinin (HA) of influenza virus. The wild-type gene has previously been shown to be expressed with high efficiency from simian virus 40 (SV40)-HA recombinant vectors into a fully glycosylated protein that is displayed on the infected cell's surface in an antigenically and biologically active form. The anchor-minus HA also is glycosylated but is secreted efficiently into the medium. By contrast, the signal-minus HA is produced only at low levels, is not glycosylated and is located intracellularly.
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